Journal: Cell reports

Article Title: Dysregulation of the mesoprefrontal dopamine circuit mediates an early-life stress-induced synaptic imbalance in the prefrontal cortex

doi: 10.1016/j.celrep.2021.109074

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-TH primary antibody Sigma-Aldrich Cat# T8700; RRID: AB_1080430 Cy3-conjugated goat anti-rabbit IgG Jackson ImmunoResearch Laboratories Cat# 111-165-003; RRID: AB_2338000 Bacterial and virus strains pAAV-CAG-tdTomato Gift from Edward Boyden Addgene# 59462 AAV1-CAG-Flex-tdTomato-WPRE-bGH Gift from Hongkui Zeng Addgene# 51503 Chemicals, peptides, and recombinant proteins Green Retrobeads Lumafluor Green Retrobeads IX Tetrodotoxin citrate Tocris Cat# 1069 (R)-CPP Tocris Cat# 0247 D-APV Tocris Cat# 0106 Bicuculline methochloride Tocris Cat# 0131 NBQX Tocris Cat# 0373 Haloperidol hydrochloride Tocris Cat# 0931 SCH23390 hydrochloride Tocris Cat# 0925 Eticlopride hydrochloride Tocris Cat# 1847 (-)-Quinpirole hydrochloride Tocris Cat# 1061 6-OHDA Tocris Cat# 2547 Experimental models: Organisms/strains Mouse: C57BL/6NJ Jackson Laboratories 005304 Mouse: DAT-IRES-Cre Jackson Laboratories 006660 Software and algorithms ImageJ NIH https://imagej.nih.gov/ij/ pClamp Molecular Devices N/A Prairie View Bruker N/A MATLAB MathWorks N/A EthoVision XT Noldus N/A Any-maze video tracking software Stoelting Co N/A Other Multiphoton microscope Bruker https://www.bruker.com/en.html Ti::sapphire laser Spectra Physics MaiTai HP 10% normal goat serum Thermo Fisher Cat# 50062Z Triton X-100 Sigma-Aldrich Cat# T8787 Open in a separate window KEY RESOURCES TABLE Early-life stress (ELS) enhances excitation-inhibition ratio in prefrontal cortex (PFC) ELS increases intrinsic excitability of PFC-projecting VTA dopaminergic neurons ELS-induced alterations in the developing PFC and VTA are long lasting Dopamine D2 receptor signaling mediates ELS-induced synaptic change in the PFC

Techniques: Virus, Recombinant, Software, Microscopy